Synchrotron SAXS
data from solutions of
Nucleolar RNA helicase 2 (DDX21) fragment 186-783 monomeric mutant
in
20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH 7.5
were collected
on the
BM29 beam line
at the ESRF storage ring
(Grenoble, France)
using a Pilatus 1M detector
at a sample-detector distance of 2.9 m and
at a wavelength of λ = 0.099 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample
at 10 mg/ml was injected at a 0.50 ml/min flow rate
onto a GE Superose 6 Increase 10/300 column
at 20°C.
2160 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
s, nm-1
