| MWI(0) | 58 | kDa |
| MWexpected | 57 | kDa |
| VPorod | 98 | nm3 |
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log I(s)
2.98×101
2.98×100
2.98×10-1
2.98×10-2
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s, nm-1
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Insights on the dynamic behavior of protein disulfide isomerase in the solution environment through the SAXS technique
Sanyasi C, Balakrishnan S, Chinnasamy T, Venugopalan N, Kandavelu P, Batra-Safferling R, Muthuvel S, In Silico Pharmacology 12(1) (2024) DOISASDGZ9 – Protein Disulfide Isomerase (PDI) purified from bovine liver
Protein disulfide-isomerase|
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Fits and models
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Synchrotron SAXS data from solutions of Protein Disulfide Isomerase (PDI) in 50 mM Tris-HCL, 150 mM NaCl, pH 7.5 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 3.5 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 2.5 and 10 mg/ml were measured at 10°C. Eight successive 1 second frames were collected. Using the automated data processing pipeline at the beam line, the data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve. Subsequent data processing and three-dimensional modeling were performed using the ATSAS [2.8.3] package.
PDI was purified from bovine liver and was incubated with 0.5mM TCEP to reduce the active sites one hour before SAXS data collection. |
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