Rapid screening of in cellulo grown protein crystals via a small-angle X-ray scattering/X-ray powder diffraction synergistic approach

Lahey-Rudolph J, Schönherr R, Jeffries C, Blanchet C, Boger J, Ferreira Ramos A, Riekehr W, Triandafillidis D, Valmas A, Margiolaki I, Svergun D, Redecke L, Journal of Applied Crystallography 53(5) (2020) DOI

SASDH36 – In cellulo Woronin body major protein (HEX-1) crystals recombinantly expressed within High Five insect cells

Woronin body major protein
MWexperimental 19 kDa
MWexpected 19 kDa
log I(s) 3.50×105 3.50×104 3.50×103 3.50×102
Woronin body major protein small angle scattering data  s, nm-1
(sRg)2I(s)/I(0)
Woronin body major protein Kratky plot 1.104 0 √3 sRg

Data validation

There are no models related to this curve.

Synchrotron SAXS data from High Five cell cultures containing in cellulo HEX-1 protein crystals were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Data were collected at 7°C using a fixed sample position (no sample flow) in a 1.8 mm capillary. 40 successive 0.045 second frames were collected; the individual 2D images were summed (1.8 s total exposure) and then normalized to the intensity of the transmitted beam and radially averaged. The scattering of the solvent-blank was subtracted.

The High Five cell cultures were suspended in TBS (20 mM Tris, 150 mM NaCl), pH 7, that was used for solvent-background scattering subtraction. Data validation metrics do not apply for this entry (Rg, I(0), MW, etc). The quoted 'experimental MW' is that of the monomeric protein calculated from the amino acid sequence.

Woronin body major protein (HEX-1)
Mol. type   Protein
Organism   Neurospora crassa
Olig. state   Unknown
Mon. MW   19.2 kDa
 
UniProt   P87252 (1-176)
Sequence   FASTA