Hierarchical Conformational Dynamics Confers Thermal Adaptability to preQ1 RNA Riboswitches.

Gong Z, Yang S, Dong X, Yang QF, Zhu YL, Xiao Y, Tang C, J Mol Biol 432(16):4523-4543 (2020) Europe PMC

SASDH38 – Wildtype preQ1 riboswitch in Bacillus subtilis, apo-state - Bsu-WT-apo

wildtype preQ1 riboswitch in Bacillus subtilis
MWexperimental 11 kDa
MWexpected 11 kDa
VPorod 20 nm3
log I(s) 3.38×101 3.38×100 3.38×10-1 3.38×10-2
wildtype preQ1 riboswitch in Bacillus subtilis small angle scattering data  s, nm-1
ln I(s)
wildtype preQ1 riboswitch in Bacillus subtilis Guinier plot ln 3.39×101 Rg: 2.6 nm 0 (2.6 nm)-2 s2
(sRg)2I(s)/I(0)
wildtype preQ1 riboswitch in Bacillus subtilis Kratky plot 1.104 0 √3 sRg
Dmax: 10.5 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Bsu-WT-apo in 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH 6.5 were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facility (SSRF; Shanghai, China) using a Pilatus 1M detector at a wavelength of λ = 0.1037 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). 20 successive 1 second frames were collected at 25°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Apo state sampling at 298K

wildtype preQ1 riboswitch in Bacillus subtilis (Bsu-WT)
Mol. type   RNA
Organism   Bacillus subtilis
Olig. state   Monomer
Mon. MW   10.9 kDa
Sequence   FASTA