Rapid screening of in cellulo grown protein crystals via a small-angle X-ray scattering/X-ray powder diffraction synergistic approach

Lahey-Rudolph J, Schönherr R, Jeffries C, Blanchet C, Boger J, Ferreira Ramos A, Riekehr W, Triandafillidis D, Valmas A, Margiolaki I, Svergun D, Redecke L, Journal of Applied Crystallography 53(5) (2020) DOI

SASDH56 – High Five insect cell culture (control; uninfected)

High Five insect cells
MWexperimental 0 kDa
MWexpected 0 kDa
log I(s) 3.50×105 3.50×104 3.50×103 3.50×102
High Five insect cells small angle scattering data  s, nm-1
(sRg)2I(s)/I(0)
High Five insect cells Kratky plot 1.104 0 √3 sRg

Data validation

There are no models related to this curve.

Synchrotron SAXS data from High Five cell cultures suspended in TBS (20 mM Tris, 150 mM NaCl), pH 7, were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Data were collected at 7°C using a fixed sample position (no sample flow) in a 1.8 mm capillary. 40 successive 0.045 second frames were collected; the individual 2D images were summed (1.8 s total exposure) and then normalized to the intensity of the transmitted beam and radially averaged. The scattering of the solvent-blank was subtracted.

Data validation metrics do not apply for this entry (Rg, I(0), MW, etc).

Tags: softmatter
High Five insect cells
Mol. type   Other
Organism   Trichoplusia ni
Olig. state   Unknown
Mon. MW   0.0 kDa
Chemical formula