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X-ray synchrotron radiation scattering data from the from the chemically cross-linked complex of the beta isoform of the 14-3-3 protein and ARHGAP22 in 25 mM HEPES 150mM NaCl pH 7.5 were collected on the SAXS/WAXS beam line of the Australian Synchrotron (Melbourne, Australia) using a 2D Photon counting Pilatus 1M-W pixel detector (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle; λ=0.10332 nm). For both the sample and buffer, five successive 2 second frames were collected from ~80 ul of protein flowed past the beam, where the protein concentration was 0.35 mg/ml. The data were normalized to the intensity of the transmitted beam, place on an absolute scale against water, radially averaged and the scattering of the solvent-blank was subtracted. The models and corresponding fits are from a model optimised using CORAL. Of note, the intensity error estimates are given as 2-standard errors, thus, the Chi values are a factor of two times lower in the log files than is actually the case. Data were also collected on a titration series of the non-cross linked complex, and were analysed to determine an approximate equilibrium constant. This analysis can be found in the attached zip archive.
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s, nm-1
