| MWI(0) | 94 | kDa |
| MWexpected | 87 | kDa |
|
log I(s)
2.89×101
2.89×100
2.89×10-1
2.89×10-2
|
s, nm-1
|
Structural basis of client specificity in mitochondrial membrane-protein chaperones.
Sučec I, Wang Y, Dakhlaoui O, Weinhäupl K, Jores T, Costa D, Hessel A, Brennich M, Rapaport D, Lindorff-Larsen K, Bersch B, Schanda P, Sci Adv 6(51) (2020) Europe PMCSASDH89 – Mitochondrial TIM8·13 chaperone in complex with the Tim23 membrane protein precursor
Mitochondrial import inner membrane translocase subunit TIM8Mitochondrial import inner membrane translocase subunit TIM13
Mitochondrial import inner membrane translocase subunit TIM23
|
|
|
Fits and models
|
|
|
Synchrotron SAXS
data from solutions of
Mitochondrial TIM8·13 chaperone in complex with the Tim23 membrane protein precursor
in
50 mM Tris, 150 mM NaCl, pH 7.4
were collected
on the
BM29 beam line
at the ESRF storage ring
(Grenoble, France)
using a Pilatus 1M detector
at a sample-detector distance of 2.9 m and
at a wavelength of λ = 0.099 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 500.00 μl sample
at 2.7 mg/ml was injected at a 0.20 ml/min flow rate
onto a GE Superdex 200 10/300 column
at 20°C.
21 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
|
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||