| MWexperimental | 131 | kDa |
| MWexpected | 147 | kDa |
| VPorod | 218 | nm3 |
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log I(s)
1.45×102
1.45×101
1.45×100
1.45×10-1
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s, nm-1
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Allosteric activation of the nitric oxide receptor soluble guanylate cyclase mapped by cryo-electron microscopy.
Horst BG, Yokom AL, Rosenberg DJ, Morris KL, Hammel M, Hurley JH, Marletta MA, Elife 8 (2019) Europe PMCSASDHB2 – Soluble guanylate cyclase (nitric oxide, NO, bound)
Soluble guanylyl cyclase alpha-1 subunitSoluble guanylyl cyclase beta-1 subunit
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Fits and models
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Synchrotron SAXS data from solutions of guanylate cyclase (NO bound) in 50 mM KH2PO4,150 mM NaCl, 2% glycerol, 500 µM NO, pH 7.4 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 60.00 μl sample at 7 mg/ml was injected at a 0.45 ml/min flow rate onto a Shodex KW-800 series column at 20°C. 20 successive 3 second frames were collected through the SEC elution peak of the sample. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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