| MWexperimental | 12 | kDa |
| MWexpected | 9 | kDa |
| VPorod | 43 | nm3 |
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log I(s)
2.70×10-2
2.70×10-3
2.70×10-4
2.70×10-5
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s, nm-1
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The BRCA2-MEILB2-BRME1 complex governs meiotic recombination and impairs the mitotic BRCA2-RAD51 function in cancer cells
Zhang J, Gurusaran M, Fujiwara Y, Zhang K, Echbarthi M, Vorontsov E, Guo R, Pendlebury D, Alam I, Livera G, Emmanuelle M, Wang P, Nandakumar J, Davies O, Shibuya H, Nature Communications 11(1) (2020) DOISASDHC9 – Meiotic localizer of BRCA2 - MEILB1a2 monomer
Meiotic localizer of BRCA2|
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Fits and models
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Synchrotron SAXS data from solutions of the meiotic localizer of BRCA2 (MEILB1a2 monomer) in 20 mM Tris pH 8.0, 150 mM KCl were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Pilatus 2M detector at a wavelength of λ = 0.09524 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100 μl sample at 10 mg/ml was injected at a 0.50 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 20 successive 3 second frames were collected through the sample elution peak. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of an appropriate solvent-blank from the SEC-elution was subtracted.
Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN |
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