SASDHE4 – Trimeric complex of myosin A with myosin tail interacting protein (MTIP, residues 60-204) and essential light chain (PfELC) from Plasmodium falciparum
Synchrotron SAXS data from solutions of the trimeric complex between myosin A, the myosin tail interacting protein (MTIP, residues 60-204) and the essential light chain (PfELC) from Plasmodium falciparum in 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP were collected on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 90.00 μl sample at 8.2 mg/ml was injected at a 0.80 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 1500 successive 0.995 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
s, nm-1
