Zinc-finger protein CNBP alters the 3-D structure of lncRNA Braveheart in solution

Kim D, Thiel B, Mrozowich T, Hennelly S, Hofacker I, Patel T, Sanbonmatsu K, Nature Communications 11(1) (2020) DOI

SASDHH3 – Braveheart 3' module in 6 mM MgCl2

Braveheart 3' module
MWexperimental 72 kDa
MWexpected 72 kDa
VPorod 198 nm3
log I(s) 3.40×10-2 3.40×10-3 3.40×10-4 3.40×10-5
Braveheart 3' module small angle scattering data  s, nm-1
ln I(s)
Braveheart 3' module Guinier plot ln 3.40×10-2 Rg: 6.5 nm 0 (6.5 nm)-2 s2
(sRg)2I(s)/I(0)
Braveheart 3' module Kratky plot 1.104 0 √3 sRg
p(r)
Braveheart 3' module pair distance distribution function Rg: 6.1 nm 0 Dmax: 18.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Braveheart 3' module DAMMIN model
log I(s)
 s, nm-1
Braveheart 3' module DAMMIN model
log I(s)
 s, nm-1
Braveheart 3' module DAMMIN model
Synchrotron SAXS data from solutions of Braveheart 3' module in 6 mM MgCl2 in 50 mM HEPES-KOH, 100 mM KCl, 6 mM MgCl2, pH 7.6 were collected on the B21 beam line at the Diamond Light Source storage ring (Didcot, UK) using a Pilatus 2M detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A sample at 1.4 mg/ml was injected onto a column . 620 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

This fragment contains nucleotides 315 to 538 of Braveheart RNA. SEC-column type and SEC parameters (flow-rate, injection volume) = UNKNOWN; Experimental temperature = UNKNOWN

Braveheart 3' module (Bvht 3')
Mol. type   RNA
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   72.3 kDa
Sequence   FASTA