Zinc-finger protein CNBP alters the 3-D structure of lncRNA Braveheart in solution

Kim D, Thiel B, Mrozowich T, Hennelly S, Hofacker I, Patel T, Sanbonmatsu K, Nature Communications 11(1) (2020) DOI

SASDHK3 – Braveheart RNA + Cellular nucleic acid-binding protein (CNBP)

Braveheart RNA
Cellular nucleic acid-binding protein
MWexperimental 227 kDa
MWexpected 227 kDa
VPorod 1660 nm3
log I(s) 1.50×10-1 1.50×10-2 1.50×10-3 1.50×10-4
Braveheart RNA Cellular nucleic acid-binding protein small angle scattering data  s, nm-1
ln I(s)
Braveheart RNA Cellular nucleic acid-binding protein Guinier plot ln 1.50×10-1 Rg: 9.8 nm 0 (9.8 nm)-2 s2
(sRg)2I(s)/I(0)
Braveheart RNA Cellular nucleic acid-binding protein Kratky plot 1.104 0 3 sRg
p(r)
Braveheart RNA Cellular nucleic acid-binding protein pair distance distribution function Rg: 9.5 nm 0 Dmax: 30.2 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Braveheart RNA Cellular nucleic acid-binding protein DAMMIN model

log I(s)
 s, nm-1
Braveheart RNA Cellular nucleic acid-binding protein DAMMIN model

log I(s)
 s, nm-1
Braveheart RNA Cellular nucleic acid-binding protein DAMMIN model

Synchrotron SAXS data from solutions of Braveheart RNA + Cellular nucleic acid-binding protein (CNBP) in 50 mM HEPES-KOH, 100 mM KCl, 6 mM MgCl2, pH 7.6 were collected on the B21 beam line at the Diamond Light Source storage ring (Didcot, UK) using a Pilatus 2M detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A sample at 0.9 mg/ml was injected onto a column . 620 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

This sample is the complex of Braveheart RNA and CNBP protein. SEC-column type and SEC parameters (flow-rate, injection volume) = UNKNOWN; Experimental temperature = UNKNOWN

Braveheart RNA (Bvht)
Mol. type   RNA
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   205.4 kDa
Sequence   FASTA
 
Cellular nucleic acid-binding protein (CNBP)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   21.5 kDa
 
UniProt   P62633 (1-177)
Sequence   FASTA