Zinc-finger protein CNBP alters the 3-D structure of lncRNA Braveheart in solution

Kim D, Thiel B, Mrozowich T, Hennelly S, Hofacker I, Patel T, Sanbonmatsu K, Nature Communications 11(1) (2020) DOI

SASDHL3 – Braveheart Fragment 1 + Cellular nucleic acid-binding protein (CNBP)

Cellular nucleic acid-binding protein
Braveheart Fragment 1
MWexperimental 137 kDa
MWexpected 137 kDa
VPorod 455 nm3
log I(s) 1.06×10-1 1.06×10-2 1.06×10-3 1.06×10-4
Cellular nucleic acid-binding protein Braveheart Fragment 1 small angle scattering data  s, nm-1
ln I(s)
Cellular nucleic acid-binding protein Braveheart Fragment 1 Guinier plot ln 1.06×10-1 Rg: 8.2 nm 0 (8.2 nm)-2 s2
(sRg)2I(s)/I(0)
Cellular nucleic acid-binding protein Braveheart Fragment 1 Kratky plot 1.104 0 √3 sRg
p(r)
Cellular nucleic acid-binding protein Braveheart Fragment 1 pair distance distribution function Rg: 8.2 nm 0 Dmax: 27 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Cellular nucleic acid-binding protein Braveheart Fragment 1 DAMMIN model
log I(s)
 s, nm-1
Cellular nucleic acid-binding protein Braveheart Fragment 1 DAMMIN model
log I(s)
 s, nm-1
Cellular nucleic acid-binding protein Braveheart Fragment 1 DAMMIN model
Synchrotron SAXS data from solutions of Braveheart Fragment 1 + Cellular nucleic acid-binding protein (CNBP) in 50 mM HEPES-KOH, 100 mM KCl, 6 mM MgCl2, pH 7.6 were collected on the B21 beam line at the Diamond Light Source storage ring (Didcot, UK) using a Pilatus 2M detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A sample at 2.5 mg/ml was injected onto a column . 620 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

This sample contains nucleotides 1-358 of Braveheart RNA and CNBP protein. SEC-column type and SEC parameters (flow-rate, injection volume) = UNKNOWN; Experimental temperature = UNKNOWN

Cellular nucleic acid-binding protein (CNBP)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   21.5 kDa
 
UniProt   P62633 (1-177)
Sequence   FASTA
 
Braveheart Fragment 1 (Bvht F1)
Mol. type   RNA
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   116.0 kDa
Sequence   FASTA