Allosteric regulation of lysosomal enzyme recognition by the cation-independent mannose 6-phosphate receptor.

Olson LJ, Misra SK, Ishihara M, Battaile KP, Grant OC, Sood A, Woods RJ, Kim JP, Tiemeyer M, Ren G, Sharp JS, Dahms NM, Commun Biol 3(1):498 (2020) Europe PMC

SASDHL4 – N-terminal domains 1-5 of the cation-independent mannose-6-phosphate receptor (CI-MPR)

Cation-independent mannose-6-phosphate receptor
MWI(0) 88 kDa
MWexpected 81 kDa
VPorod 140 nm3
log I(s) 1.29×101 1.29×100 1.29×10-1 1.29×10-2
Cation-independent mannose-6-phosphate receptor small angle scattering data  s, nm-1
ln I(s)
Cation-independent mannose-6-phosphate receptor Guinier plot ln 1.29×101 Rg: 3.7 nm 0 (3.7 nm)-2 s2
(sRg)2I(s)/I(0)
Cation-independent mannose-6-phosphate receptor Kratky plot 1.104 0 √3 sRg
p(r)
Cation-independent mannose-6-phosphate receptor pair distance distribution function Rg: 3.6 nm 0 Dmax: 10.1 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of the CI-MPR (N-terminal domains 1-5) in 20 mM imidazole, 150 mM NaCl, 5 mM beta glycerol phosphate, 10 mM MnCl2, pH 6.4 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 3.5 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 22°C. 16 successive 0.690 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Cation-independent mannose-6-phosphate receptor (CI-MPR)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   81.0 kDa
 
UniProt   P11717 (36-761)
Sequence   FASTA