Salt Dependence of A-Form RNA Duplexes: Structures and Implications.

Chen YL, Pollack L, J Phys Chem B 123(46):9773-9785 (2019) Europe PMC

SASDHP2 – 12 base-paired RNA double helix (RNA12) with 5 mM MgCl2 - SWAXS

12 base-paired RNA double helix
MWexperimental 8 kDa
MWexpected 8 kDa
log I(s) 3.57×10-2 3.57×10-3 3.57×10-4 3.57×10-5
12 base-paired RNA double helix small angle scattering data  s, nm-1
ln I(s)
12 base-paired RNA double helix Guinier plot ln 3.58×10-2 Rg: 2.0 nm 0 (2.0 nm)-2 s2
(sRg)2I(s)/I(0)
12 base-paired RNA double helix Kratky plot 1.104 0 √3 sRg

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of RNA12 in 5 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH 7 were collected on the G1 beam line at the Cornell High Energy Synchrotron Source (CHESS) storage ring (Ithaca, NY, USA) using two PILATUS 100K detectors at a sample-detector distances of 0.45 and 1.7 m and at a wavelength of λ = 0.1246 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.53 mg/ml was measured at 20°C. 60 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the data merged from the two detector positions.

12 base-paired RNA double helix (RNA12)
Mol. type   RNA
Olig. state   Monomer
Mon. MW   7.7 kDa
Sequence   FASTA