The hTERT core promoter forms three parallel G-quadruplexes.

Monsen RC, DeLeeuw L, Dean WL, Gray RD, Sabo TM, Chakravarthy S, Chaires JB, Trent JO, Nucleic Acids Res (2020) Europe PMC

SASDHQ3 – Human Telomerase Reverse Transcriptase Core Promoter (hTERT) PQS2-PQS3 region, WT_PQS23

hTERT core promoter PQS2-PQS3 region
MWexperimental 13 kDa
MWexpected 14 kDa
VPorod 18 nm3
log I(s) 1.30×10-3 1.30×10-4 1.30×10-5 1.30×10-6
hTERT core promoter PQS2-PQS3 region small angle scattering data  s, nm-1
ln I(s)
hTERT core promoter PQS2-PQS3 region Guinier plot ln 1.31×10-3 Rg: 1.7 nm 0 (1.7 nm)-2 s2
(sRg)2I(s)/I(0)
hTERT core promoter PQS2-PQS3 region Kratky plot 1.104 0 √3 sRg
p(r)
hTERT core promoter PQS2-PQS3 region pair distance distribution function Rg: 1.7 nm 0 Dmax: 5.4 nm

Data validation

Fits and models

log I(s)
 s, nm-1
hTERT core promoter PQS2-PQS3 region DAMFILT model
Synchrotron SAXS data from solutions of WT_PQS23 in 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH 7.2 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 3.5 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 250 μl sample at 5.2 mg/ml was injected at a 0.75 ml/min flow rate onto a GE Superdex 75 Increase 10/300 column at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Number of frames = UNKNOWN

hTERT core promoter PQS2-PQS3 region (WT PQS23)
Mol. type   DNA
Organism   synthetic construct
Olig. state   Monomer
Mon. MW   14.4 kDa
Sequence   FASTA