Salt Dependence of A-Form RNA Duplexes: Structures and Implications.

Chen YL, Pollack L, J Phys Chem B 123(46):9773-9785 (2019) Europe PMC

SASDHR2 – 25 base-paired RNA double helix (RNA25) with 10 mM MgCl2 - WAXS

25 base-paired RNA double helix
MWexperimental 18 kDa
MWexpected 16 kDa
log I(s) 4.05×103 4.05×102 4.05×101 4.05×100
25 base-paired RNA double helix small angle scattering data  s, nm-1
ln I(s)
25 base-paired RNA double helix Guinier plot ln 4.06×103 Rg: 2.6 nm 0 (2.6 nm)-2 s2
(sRg)2I(s)/I(0)
25 base-paired RNA double helix Kratky plot 1.104 0 √3 sRg

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of RNA25 in 10 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH 7 were collected on the G1 beam line at the Cornell High Energy Synchrotron Source (CHESS) storage ring (Ithaca, NY, USA) using a Eiger X 1M detector at a sample-detector distance of 0.4 m and at a wavelength of λ = 0.1246 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.61 mg/ml was measured at 20°C. 40 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

25 base-paired RNA double helix (RNA25)
Mol. type   RNA
Olig. state   Monomer
Mon. MW   16.1 kDa
Sequence   FASTA