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Synchrotron SAXS data from solutions of Cqm1 protein in PBS buffer (10 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl), pH 7.4 were collected on the BL-18 beam line at INDUS-2 (Indore, India) using a MAR 345 Image Plate detector at a sample-detector distance of 32.4 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.60 mg/ml was measured at 25°C. One 600 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Low q (< 0.02 A-1) and high q > 0.25 A-1) experimental SAXS points were removed prior to ATSAS analysis. The protein exists as a dimer in solution (PDB ID, 6K5P_AB). SAXS data is on relative scale, thus concentration normalization and I(0) for MW estimation has not been done. CAUTION! Error propagation likely incorrect! CAUTION! Errors are severely over estimated. CAUTION! Buffer mismatch likely. CAUTION! Porod volume is inconsistent with a protein MW of 129 kDa. CAUTION! p(r) profile does not fit the SAXS data. CAUTION! Error on Rg (from Guinier) is in excess of 1 nm. CAUTION! sRg range is limited to 0.83 < sRg < 1.21. CAUTION! DAM model representation is derived from individual model spatial alignment and filtering (DAMFILT.pdb); individual DAM model fits not provided; NSD unknown.
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s, nm-1
