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Synchrotron SAXS data from solutions of carbonic anhydrase IX in 50 mM Tris, 150 mM NaCl, pH 7.5 were collected on the G1 beam line at the Cornell High Energy Synchrotron Source (CHESS; Ithaca, NY, USA) using a Pilatus 100K detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.1245 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 150.00 μl sample at 1.5 mg/ml was injected at a 0.30 ml/min flow rate onto a GE Superdex 200 5/150 column at 4°C. 799 successive 1 second frames were collected through the SEC elution. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted from selected sample peak frames.
The Ensemble Optimization Method (EOM) results that include model representatives from the refined ensemble and the corresponding volume fractions, are made available in the full entry zip archive.
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s, nm-1
Rg, nm
