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Synchrotron SAXS data from solutions of the BinB/Cqm1 complex in PBS buffer (10 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl), pH 7.4 were collected on the BL-18 beam line at INDUS-2 (Indore, India) using a MAR 345 Image Plate detector at a sample-detector distance of 32.4 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One 600 second frame was collected at 25°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The entry is for the synchrotron SAXS data on the complex of Cqm1 and BinB proteins. BinB is the receptor binding component of mosquito-larvicidal BinAB toxin and Cqm1 is its receptor from Culex mosquito. The two proteins interact with high affinity (equilibrium dissociation constant, Kd, 10 nM).
Experimental data points of low q ( < 0.02 A-1) and high q (> 0.25 A-1) were removed prior to ATSAS analysis. The data is on relative scale. Normalization to unit protein concentration and estimation of I(0) for MW estimation is thus not done. The atomic model of the complex was derived from computational docking with SAXS restraints. CAUTION! Error propagation likely incorrect! CAUTION! Errors are severely over estimated. CAUTION! p(r) profile does not fit the SAXS data. CAUTION! Buffer mismatch likely. CAUTION! Error on Rg (from Guinier) is in excess of 1 nm. CAUTION! Guinier sRg range is limited to 0.93 < sRg < 1.04. CAUTION! Non-linear Guinier plot 1.11 < sRg < 1.3. CAUTION! Concentration unknown. CAUTION! DAM model representation is derived from individual model spatial alignment and filtering (DAMFILT.pdb); individual DAM model fits not provided; NSD unknown.
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s, nm-1
