Assembly of the mitochondrial Complex I assembly complex suggests a regulatory role for deflavination.

Giachin G, Jessop M, Bouverot R, Acajjaoui S, Saidi M, Chretien A, Bacia-Verloop M, Signor L, Mas PJ, Favier A, Borel Meneroud E, Hons M, Hart DJ, Kandiah E, Boeri Erba E, Buisson A, Leonard G, Gutsche I, Soler-Lopez M, Angew Chem Int Ed Engl (2020) Europe PMC

SASDHU4 – ECSIT C-terminal domain

Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial
MWI(0) 46 kDa
MWexpected 44 kDa
VPorod 117 nm3
log I(s) 5.32×101 5.32×100 5.32×10-1 5.32×10-2
Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial small angle scattering data  s, nm-1
ln I(s)
Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial Guinier plot ln 5.32×101 Rg: 3.7 nm 0 (3.7 nm)-2 s2
(sRg)2I(s)/I(0)
Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial Kratky plot 1.104 0 √3 sRg
p(r)
Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial pair distance distribution function Rg: 3.7 nm 0 Dmax: 11.9 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial DAMMIN model
Synchrotron SAXS data from solutions of ECSIT C-terminal domain in 25 mM HEPES, 250 mM NaCl, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.8 and 4.6 mg/ml were measured at 20°C. 10 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Three different concentrations were tested (0.8, 1.2 and 4.6 mg/mL) and data were merged.

Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial (ECSIT)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   21.9 kDa
 
UniProt   Q9BQ95 (249-431)
Sequence   FASTA