| MWI(0) | 121 | kDa |
| MWexpected | 118 | kDa |
| VPorod | 174 | nm3 |
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log I(s)
8.20×10-2
8.20×10-3
8.20×10-4
8.20×10-5
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s, nm-1
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Liposome-Based Study Provides Insight into Cellular Internalization Mechanism of Mosquito-Larvicidal BinAB Toxin.
Sharma M, Kumar A, Kumar V, J Membr Biol (2020) Europe PMCSASDHU8 – SANS study of the complex between mosquito-larvicidal BinB protein and the mosquito-larvicidal binary toxin receptor (Cqm1)
Binary larvicide subunit BinBSynthetic construct (mutant, His-tagged): Mosquito-larvicidal BinAB toxin receptor protein (Neutral and basic amino acid transport protein rBAT)
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Fits and models
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SANS data from solutions of the BinB/Cqm1 complex in 25 mM HEPES, pD 7.5, 25 mM NaCl, 100% D2O, were collected using a Ingenuously built detector at a wavelength of λ = 0.52 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 25°C. One 43200 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The BinB component of larvicidal binary toxin binds receptor Cqm1 with high affinity (KD, ~10 nM). The complex migrates as a single particle on Native-PAGE. From its molecular weight and stoichiometry, the complex consists of one molecule each of Cqm1 and BinB proteins. The SANS experiments were performed using a sample reconstituted in 100% D2O. CAUTION! The corresponding model fit to the data relates to the ab initio bead model displayed in this entry and not the atomistic high-resolution model. |
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