Salt Dependence of A-Form RNA Duplexes: Structures and Implications.

Chen YL, Pollack L, J Phys Chem B 123(46):9773-9785 (2019) Europe PMC

SASDHV2 – 25 base-paired DNA double helix (DNA25) with 2 mM MgCl2 - SWAXS

25 base-paired DNA double helix
MWexperimental 16 kDa
MWexpected 15 kDa
log I(s) 6.52×10-1 6.52×10-2 6.52×10-3 6.52×10-4
25 base-paired DNA double helix small angle scattering data  s, nm-1
ln I(s)
25 base-paired DNA double helix Guinier plot ln 6.52×10-1 Rg: 1.9 nm 0 (1.9 nm)-2 s2
(sRg)2I(s)/I(0)
25 base-paired DNA double helix Kratky plot 1.104 0 √3 sRg

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of DNA25 in 2 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH 7 were collected on the G1 beam line at the Cornell High Energy Synchrotron Source (CHESS) storage ring (Ithaca, NY, USA) using a Eiger X 1M detector at a sample-detector distance of 0.4 m and at a wavelength of λ = 0.1246 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.53 mg/ml was measured at 20°C. 40 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

25 base-paired DNA double helix (DNA25)
Mol. type   DNA
Olig. state   Monomer
Mon. MW   15.5 kDa
Sequence   FASTA