Assembly of the mitochondrial Complex I assembly complex suggests a regulatory role for deflavination.

Giachin G, Jessop M, Bouverot R, Acajjaoui S, Saidi M, Chretien A, Bacia-Verloop M, Signor L, Mas PJ, Favier A, Borel Meneroud E, Hons M, Hart DJ, Kandiah E, Boeri Erba E, Buisson A, Leonard G, Gutsche I, Soler-Lopez M, Angew Chem Int Ed Engl (2020) Europe PMC

SASDHW4 – Mitochondrial Complex I assembly factor, ACAD9

Complex I assembly factor ACAD9, mitochondrial
MWI(0) 127 kDa
MWexpected 132 kDa
VPorod 217 nm3
log I(s) 7.12×101 7.12×100 7.12×10-1 7.12×10-2
Complex I assembly factor ACAD9, mitochondrial small angle scattering data  s, nm-1
ln I(s)
Complex I assembly factor ACAD9, mitochondrial Guinier plot ln 7.13×101 Rg: 3.6 nm 0 (3.6 nm)-2 s2
(sRg)2I(s)/I(0)
Complex I assembly factor ACAD9, mitochondrial Kratky plot 1.104 0 √3 sRg
p(r)
Complex I assembly factor ACAD9, mitochondrial pair distance distribution function Rg: 3.5 nm 0 Dmax: 10.2 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Complex I assembly factor ACAD9, mitochondrial DAMMIN model
Synchrotron SAXS data from solutions of ACAD9 in 25 mM HEPES, 250 mM NaCl, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 250.00 μl sample at 10.2 mg/ml was injected at a 0.50 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 60 successive 1 second frames were collected through the sample elution peak. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Complex I assembly factor ACAD9, mitochondrial (ACAD9)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   66.0 kDa
 
UniProt   Q9H845 (38-621)
Sequence   FASTA