| MWI(0) | 126 | kDa |
| MWexpected | 131 | kDa |
| VPorod | 217 | nm3 |
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log I(s)
1.42×102
1.42×101
1.42×100
1.42×10-1
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s, nm-1
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Assembly of the mitochondrial Complex I assembly complex suggests a regulatory role for deflavination.
Giachin G, Jessop M, Bouverot R, Acajjaoui S, Saidi M, Chretien A, Bacia-Verloop M, Signor L, Mas PJ, Favier A, Borel Meneroud E, Hons M, Hart DJ, Kandiah E, Boeri Erba E, Buisson A, Leonard G, Gutsche I, Soler-Lopez M, Angew Chem Int Ed Engl (2020) Europe PMCSASDHX4 – Mitochondrial complex I assembly factor, ACAD9, chimeric construct where segment 445-482 has been replaced with segment 481-518 of VLCAD protein
Complex I assembly factor ACAD9-VLCAD chimera|
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Fits and models
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Synchrotron SAXS data from solutions of an ACAD9 chimeric construct where segment 445-482 was replaced with segment 481-518 of VLCAD protein in 25 mM HEPES, 250 mM NaCl, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 250.00 μl sample at 9.1 mg/ml was injected at a 0.50 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 100 successive 1 second frames were collected through the SEC elution peak. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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