| MWexperimental | 59 | kDa |
| MWexpected | 54 | kDa |
| VPorod | 75 | nm3 |
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log I(s)
2.72×10-3
2.72×10-4
2.72×10-5
2.72×10-6
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s, nm-1
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The first DEP domain of the RhoGEF P-Rex1 autoinhibits activity andcontributes to membrane binding.
Ravala SK, Hopkins JB, Plescia CB, Allgood SR, Kane MA, Cash JN, Stahelin RV, Tesmer JJG, J Biol Chem (2020) Europe PMCSASDHX9 – Phosphorylated phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (amino acids 38-499): pDHPHDEP1
Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1|
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Fits and models
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Rg, nm
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Synchrotron SAXS
data from solutions of
Phosphorylated phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (amino acids 38-499): pDHPHDEP1
in
20 mM HEPES, 300 mM NaCl, pH 7
were collected
on the
BioCAT 18ID beam line
at the Advanced Photon Source (APS), Argonne National Laboratory storage ring
(Lemont, IL, USA)
using a Pilatus3 X 1M detector
at a sample-detector distance of 3.7 m and
at a wavelength of λ = 0.1033 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 5.00 mg/ml was measured
at 22°C.
1900 successive
0.500 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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