Membrane curvature protein exhibits interdomain flexibility and binds a small GTPase.

King GJ, Stöckli J, Hu SH, Winnen B, Duprez WG, Meoli CC, Junutula JR, Jarrott RJ, James DE, Whitten AE, Martin JL, J Biol Chem 287(49):40996-1006 (2012) Europe PMC

SASDHY2 – Human APPL2 (DCC-interacting protein 13-beta)

Adaptor protein, phosphotyrosine interaction, pleckstrin homology domain, and leucine zipper-containing protein 2
MWI(0) 91 kDa
MWexpected 87 kDa
VPorod 140 nm3
log I(s) 4.96×10-2 4.96×10-3 4.96×10-4 4.96×10-5
Adaptor protein, phosphotyrosine interaction, pleckstrin homology domain, and leucine zipper-containing protein 2 small angle scattering data  s, nm-1
ln I(s)
Adaptor protein, phosphotyrosine interaction, pleckstrin homology domain, and leucine zipper-containing protein 2 Guinier plot ln 4.96×10-2 Rg: 5.1 nm 0 (5.1 nm)-2 s2
(sRg)2I(s)/I(0)
Adaptor protein, phosphotyrosine interaction, pleckstrin homology domain, and leucine zipper-containing protein 2 Kratky plot 1.104 0 √3 sRg
p(r)
Adaptor protein, phosphotyrosine interaction, pleckstrin homology domain, and leucine zipper-containing protein 2 pair distance distribution function Rg: 5.4 nm 0 Dmax: 18 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Adaptor protein, phosphotyrosine interaction, pleckstrin homology domain, and leucine zipper-containing protein 2 BUNCH model
X-ray synchrotron radiation scattering data from APPL2 in 25 mM HEPES 150 mM NaCl, 5 mM MgCl2, 1 mM DTT pH 7.5 were collected on the SAXS/WAXS beam line of the Australian Synchrotron (Melbourne, Australia) using a 2D Photon counting Pilatus 1M-W pixel detector (s = 4π sin θ/λ, where 2θ is the scattering angle). Six successive 2 second frames were collected across solute concentrations of 0.75-2.00 mg/ml. The SAXS data displayed this entry was derived from a 0.75 mg/ml sample. The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering of the solvent-blank was subtracted. The models and corresponding fits include those derived from dummy-atom modelling using BUNCH08. Of note, the intensity error estimates are given as 2-standard error, thus, the Chi values are a factor of two times lower in the log files than is actually the case (0.567 versus 1.134).

Adaptor protein, phosphotyrosine interaction, pleckstrin homology domain, and leucine zipper-containing protein 2 (APPL2)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   43.5 kDa
 
UniProt   Q8NEU8 (1-384)
Sequence   FASTA
 
PDB ID   4H8S