Assembly of the mitochondrial Complex I assembly complex suggests a regulatory role for deflavination.

Giachin G, Jessop M, Bouverot R, Acajjaoui S, Saidi M, Chretien A, Bacia-Verloop M, Signor L, Mas PJ, Favier A, Borel Meneroud E, Hons M, Hart DJ, Kandiah E, Boeri Erba E, Buisson A, Leonard G, Gutsche I, Soler-Lopez M, Angew Chem Int Ed Engl (2020) Europe PMC

SASDHY4 – Mitochondrial very long-chain specific acyl-CoA dehydrogenase enzyme, VLCAD

Very long-chain specific acyl-CoA dehydrogenase, mitochondrial
MWI(0) 131 kDa
MWexpected 126 kDa
VPorod 215 nm3
log I(s) 1.34×102 1.34×101 1.34×100 1.34×10-1
Very long-chain specific acyl-CoA dehydrogenase, mitochondrial small angle scattering data  s, nm-1
ln I(s)
Very long-chain specific acyl-CoA dehydrogenase, mitochondrial Guinier plot ln 1.34×102 Rg: 3.4 nm 0 (3.4 nm)-2 s2
(sRg)2I(s)/I(0)
Very long-chain specific acyl-CoA dehydrogenase, mitochondrial Kratky plot 1.104 0 √3 sRg
p(r)
Very long-chain specific acyl-CoA dehydrogenase, mitochondrial pair distance distribution function Rg: 3.3 nm 0 Dmax: 9.9 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Very long-chain specific acyl-CoA dehydrogenase, mitochondrial DAMFILT model
Very long-chain specific acyl-CoA dehydrogenase, mitochondrial DAMMIF model
Synchrotron SAXS data from solutions of VLCAD in 25 mM HEPES, 250 mM NaCl, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 250.00 μl sample at 8.8 mg/ml was injected at a 0.50 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 100 successive 1 second frames were collected through the SEC elution peak. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Very long-chain specific acyl-CoA dehydrogenase, mitochondrial (VLCAD)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   63.0 kDa
 
UniProt   P49748 (72-655)
Sequence   FASTA