Synchrotron SAXS
data from solutions of
Escherichia coli RnlA (mRNA endoribonuclease toxin LS) - R255A single alanine mutant
in
20 mM Tris, 150 mM NaCl, 1 mM TCEP, 5% glycerol, pH 8
were collected
on the
BM29 beam line
at the ESRF storage ring
(Grenoble, France)
using a Pilatus 1M detector
at a sample-detector distance of 2.8 m and
at a wavelength of λ = 0.09919 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample
at 13 mg/ml was injected at a 0.20 ml/min flow rate
onto a Shodex KW402.5-4F column
at 20°C.
900 successive
2 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
s, nm-1
