Molecular flexibility of the condensin subunit Ycs4 is modulated by kleisin binding

Karen Manalastas-Cantos.

SASDHZ9 – Condensin complex subunit 1, Ycs4

Condensin complex subunit 1
MWI(0) 142 kDa
MWexpected 137 kDa
VPorod 296 nm3
log I(s) 1.45×104 1.45×103 1.45×102 1.45×101
Condensin complex subunit 1 small angle scattering data  s, nm-1
ln I(s)
Condensin complex subunit 1 Guinier plot ln 1.46×104 Rg: 5.2 nm 0 (5.2 nm)-2 s2
(sRg)2I(s)/I(0)
Condensin complex subunit 1 Kratky plot 1.104 0 √3 sRg
p(r)
Condensin complex subunit 1 pair distance distribution function Rg: 5.4 nm 0 Dmax: 17.7 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Condensin complex subunit 1 MONSA model
log I(s)
 s, nm-1
Condensin complex subunit 1 SREFLEX model
Synchrotron SAXS data from solutions of Ycs4 in 25 mM Tris, 300 mM NaCl, 1mM DTT, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.5 and 10 mg/ml were measured at 20°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Condensin complex subunit 1 (Ycs4)
Mol. type   Protein
Organism   Chaetomium thermophilum
Olig. state   Monomer
Mon. MW   137.0 kDa
 
UniProt   G0SB82 (3-1222)
Sequence   FASTA
 
PDB ID   6QJ3