| MWI(0) | 150 | kDa |
| MWexpected | 146 | kDa |
| VPorod | 279 | nm3 |
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log I(s)
1.54×104
1.54×103
1.54×102
1.54×101
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s, nm-1
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Molecular flexibility of the condensin subunit Ycs4 is modulated by kleisin binding
Karen Manalastas-Cantos.SASDJ22 – Condensin complex subunit 1 (Ycs4) bound to condensin complex subunit 2, 336-418 (Brn1S)
Condensin complex subunit 1Condensin complex subunit 2, 336-418
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Fits and models
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Synchrotron SAXS data from solutions of the Ycs4-Brn1S complex in 25 mM Tris, 300 mM NaCl, 1mM DTT, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.5 and 5 mg/ml were measured at 20°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
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