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Synchrotron SAXS data from solutions of Human D9-10 protein deglycosylated with a 100 fold excess of mannose-6-phosphate in 25 mM Tris 150 mM NaCl, pH 7.5, were collected using in-line SEC-SAXS at the B21 beam line at the Diamond Light Source (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 4.0 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In line SEC-SAXS was performed using an Agilent 1200 HPLC system equipped with a GE Superdex 200 Increase 3.2/300 column at 25°C. A 45 μl sample at 6.5 mg/ml was loaded onto the column at a flow rate of 0.075 mL/min. Data frames were collected for the entire column elution with an exposure time of 3 seconds per frame (620 frames total). The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted using data frames measured from the flow-through column buffer with no macromolecular sample present.
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s, nm-1
