Notch-Jagged signaling complex defined by an interaction mosaic.

Zeronian MR, Klykov O, Portell I de Montserrat J, Konijnenberg MJ, Gaur A, Scheltema RA, Janssen BJC, Proc Natl Acad Sci U S A 118(30) (2021) Europe PMC

SASDJ38 – Monomeric Jagged1 high-affinity full ectodomain

Protein jagged-1
MWexperimental 137 kDa
MWexpected 115 kDa
VPorod 375 nm3
log I(s) 7.04×10-2 7.04×10-3 7.04×10-4 7.04×10-5
Protein jagged-1 small angle scattering data  s, nm-1
ln I(s)
Protein jagged-1 Guinier plot ln 7.04×10-2 Rg: 7.4 nm 0 (7.4 nm)-2 s2
(sRg)2I(s)/I(0)
Protein jagged-1 Kratky plot 1.104 0 √3 sRg
p(r)
Protein jagged-1 pair distance distribution function Rg: 7.4 nm 0 Dmax: 24 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Monomeric Jagged1 high-affinity full ectodomain in 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH 7.4 were collected on the B21 beam line at the Diamond Light Source storage ring (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 4.0 m and (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 45.00 μl sample at 5.9 mg/ml was injected onto a column . 620 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The expected molecular weight of the protein quoted above excludes the contribution of N- and O-linked glycans. The total expected molecular weight of the protein in a monomeric state is 130 kDa (protein, 115 kDa plus glycans, 15 kDa). X-ray wavelength: UNKNOWN. Temperature: UNKNOWN. SEC column: UNKNOWN. SEC flow-rate: UNKNOWN.

Protein jagged-1
Mol. type   Protein
Organism   Mus musculus
Olig. state   Monomer
Mon. MW   114.6 kDa
 
UniProt   Q9QXX0 (31-1067)
Sequence   FASTA