Notch-Jagged signaling complex defined by an interaction mosaic.

Zeronian MR, Klykov O, Portell I de Montserrat J, Konijnenberg MJ, Gaur A, Scheltema RA, Janssen BJC, Proc Natl Acad Sci U S A 118(30) (2021) Europe PMC

SASDJ48 – Jagged1 high-affinity full ectodomain 5.3 µM

Protein jagged-1
MWexperimental 187 kDa
MWexpected 115 kDa
VPorod 430 nm3
log I(s) 1.20×10-1 1.20×10-2 1.20×10-3 1.20×10-4
Protein jagged-1 small angle scattering data  s, nm-1
ln I(s)
Protein jagged-1 Guinier plot ln 1.20×10-1 Rg: 8.1 nm 0 (8.1 nm)-2 s2
(sRg)2I(s)/I(0)
Protein jagged-1 Kratky plot 1.104 0 √3 sRg
p(r)
Protein jagged-1 pair distance distribution function Rg: 8.5 nm 0 Dmax: 30 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Jagged1 high-affinity full ectodomain 5.3 µM in 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH 7.4 were collected on the B21 beam line at the Diamond Light Source storage ring (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 4.0 m and (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.74 mg/ml was measured at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The expected molecular weight of the protein quoted above excludes the contribution of N- and O-linked glycans. The total expected molecular weight of the protein in a monomeric state is 130 kDa (protein, 115 kDa plus glycans, 15 kDa). X-ray wavelength: UNKNOWN. Exposure time = UNKNOWN.

Protein jagged-1
Mol. type   Protein
Organism   Mus musculus
Olig. state   Unknown
Mon. MW   114.6 kDa
 
UniProt   Q9QXX0 (31-1067)
Sequence   FASTA