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Synchrotron SAXS
data from solutions of
GATA double strand DNA
in
50 mM MOPS, 50 mM NaCl, pH 7
were collected
on the
BM29 beam line
at the ESRF storage ring
(Grenoble, France)
using a Pilatus 1M detector
at a sample-detector distance of 2.9 m and
at a wavelength of λ = 0.09999 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 40.00 μl sample
at 1.4 mg/ml was injected at a 0.40 ml/min flow rate
onto a GE Superdex 75 Increase 5/150 column
at 20°C.
900 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The double strand portion of the GATA element has been exposed to X-ray beam at BM29 in Grenoble (ESRF, France) after having passed into a HPLC-SEC column.
We have repeated the experiment in static dilution series and the results were reproducible. For consistency with the other samples, we decided to deposit only the SEC-SAXS data.
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s, nm-1
