Apo Archaeoglobus fulgidus Argonaute protein dimerises in solution

Elena Manakova.

SASDJ75 – apo Archaeoglobus fulgidus Argonaute protein

Piwi protein AF_1318
MWI(0) 130 kDa
MWexpected 98 kDa
VPorod 174 nm3
log I(s) 9.36×10-2 9.36×10-3 9.36×10-4 9.36×10-5
Piwi protein AF_1318 small angle scattering data  s, nm-1
ln I(s)
Piwi protein AF_1318 Guinier plot ln 9.36×10-2 Rg: 3.8 nm 0 (3.8 nm)-2 s2
(sRg)2I(s)/I(0)
Piwi protein AF_1318 Kratky plot 1.104 0 √3 sRg
p(r)
Piwi protein AF_1318 pair distance distribution function Rg: 3.8 nm 0 Dmax: 12.1 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Piwi protein AF_1318 OTHER model
log I(s)
 s, nm-1
Piwi protein AF_1318 OTHER model
Synchrotron SAXS data from solutions of apo Archaeoglobus fulgidus Argonaute protein in 20 mM TrisHCl, pH 7.5, 5 mM MgCl2, 500 mM NaCl and 2 mM DTT, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.13 mg/ml was measured at 20°C. 20 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The concentration of the sample is rather low (0.13 mg/ml), because apo AfAgo at higher concentration aggregates.

Piwi protein AF_1318 (AfAgo)
Mol. type   Protein
Organism   Archaeoglobus fulgidus
Olig. state   Dimer
Mon. MW   49.2 kDa
 
UniProt   O28951 (1-427)
Sequence   FASTA
 
PDB ID   1W9H