| MWI(0) | 28 | kDa |
| MWexpected | 22 | kDa |
| VPorod | 41 | nm3 |
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log I(s)
4.23×100
4.23×10-1
4.23×10-2
4.23×10-3
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s, nm-1
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Biophysical characterization of the complex between the iron-responsive transcription factor Fep1 and DNA.
Miele AE, Cervoni L, Le Roy A, Cutone A, Musci G, Ebel C, Bonaccorsi di Patti MC, Eur Biophys J (2021) Europe PMCSASDJ86 – Fep1 wild type
GATA-type iron responsive transcription factor Fep1|
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Fits and models
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Rg, nm
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Rg, nm
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Rg, nm
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Synchrotron SAXS
data from solutions of
Fep1 wild type
in
50 mM MOPS, 50 mM NaCl, pH 7
were collected
on the
BM29 beam line
at the ESRF storage ring
(Grenoble, France)
using a Pilatus 1M detector
at a sample-detector distance of 2.9 m and
at a wavelength of λ = 0.0999 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 40.00 μl sample
at 9.5 mg/ml was injected at a 0.40 ml/min flow rate
onto a GE Superdex 75 Increase 5/150 column
at 20°C.
900 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
SAS data derived from merging the elution peaks of 2 successive HPLC run at ESRF BM29 (Grenoble, France). Column Superdex75 5/150 (GE Healthcare) run at 20 °C, flow of 0.4 ml/min. 900 frames (1/sec) were collected per column run. Chromixs and US-SOMO HPLC-SAXS were used to convert the I(q) vs t in I(q) vs q. For the buffer subtraction the first 20 frames of the column run were merged, after inspection of absence of capillary fouling.
Tags:
idp
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