Synchrotron SAXS
data from solutions of
Trinucleosomes from Xenopus laevis (African clawed frog)
in
20 mM Tris 150 mM NaCl 1 mM EDTA 1 mM DTT 50% w/v sucrose, pH 7.5
were collected
on the
G1 beam line
at the Cornell High Energy Synchrotron Source (CHESS) storage ring
(Ithaca, NY, USA)
using a Pilatus 200K detector
at a sample-detector distance of 2.1 m and
at a wavelength of λ = 0.111 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 0.83 mg/ml was measured
at 20°C.
70 successive
5 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
s, nm-1
