Notch-Jagged signaling complex defined by an interaction mosaic.

Zeronian MR, Klykov O, Portell I de Montserrat J, Konijnenberg MJ, Gaur A, Scheltema RA, Janssen BJC, Proc Natl Acad Sci U S A 118(30) (2021) Europe PMC

SASDJ98 – Jagged1 EGF8-11 58 µM

Protein jagged-1 EGF8-11
MWexperimental 30 kDa
MWexpected 21 kDa
VPorod 31 nm3
log I(s) 5.72×10-2 5.72×10-3 5.72×10-4 5.72×10-5
Protein jagged-1 EGF8-11 small angle scattering data  s, nm-1
ln I(s)
Protein jagged-1 EGF8-11 Guinier plot ln 5.72×10-2 Rg: 3.2 nm 0 (3.2 nm)-2 s2
(sRg)2I(s)/I(0)
Protein jagged-1 EGF8-11 Kratky plot 1.104 0 √3 sRg
p(r)
Protein jagged-1 EGF8-11 pair distance distribution function Rg: 3.3 nm 0 Dmax: 11.5 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Jagged1 EGF8-11 58 µM in 20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH 7.4 were collected on the B21 beam line at the Diamond Light Source storage ring (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 4.0 m and (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.25 mg/ml was measured at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The expected molecular weight of the protein quoted above excludes the contribution of O-linked glycans. The total expected molecular weight of the protein in a monomeric state is 22 kDa (protein, 21 kDa plus glycans, 1 kDa). X-ray wavelength: UNKNOWN. Exposure time = UNKNOWN.

Protein jagged-1 EGF8-11
Mol. type   Protein
Organism   Mus musculus
Olig. state   Unknown
Mon. MW   20.6 kDa
 
UniProt   Q9QXX0 (487-665)
Sequence   FASTA