A Giant Extracellular Matrix Binding Protein of Staphylococcus epidermidis Binds Surface-Immobilized Fibronectin via a Novel Mechanism.

Büttner H, Perbandt M, Kohler T, Kikhney A, Wolters M, Christner M, Heise M, Wilde J, Weißelberg S, Both A, Betzel C, Hammerschmidt S, Svergun D, Aepfelbacher M, Rohde H, mBio 11(5) (2020) Europe PMC

SASDJA2 – S. epidermidis extracellular matrix binding protein (Embp) FG-repeats

Extracellular matrix binding protein FG-repeats
MWexperimental 72 kDa
MWexpected 85 kDa
log I(s) 5.59×103 5.59×102 5.59×101 5.59×100
Extracellular matrix binding protein FG-repeats small angle scattering data  s, nm-1
ln I(s)
Extracellular matrix binding protein FG-repeats Guinier plot ln 5.59×103 Rg: 10.7 nm 0 (10.7 nm)-2 s2
(sRg)2I(s)/I(0)
Extracellular matrix binding protein FG-repeats Kratky plot 1.104 0 √3 sRg
p(r)
Extracellular matrix binding protein FG-repeats pair distance distribution function Rg: 11.4 nm 0 Dmax: 40 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Extracellular matrix binding protein FG-repeats EOM/RANCH model
log I(s)
 s, nm-1
Extracellular matrix binding protein FG-repeats GASBOR model
Synchrotron SAXS data from solutions of S. epidermidis extracellular matrix binding protein (Embp) FG-repeats in PBS, 136.5 mM NaCl, 2.65 mM KCl, 8.3 mM Na2HPO4, 2.65 mM KH2PO4, pH 7.4 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 10.00 mg/ml was measured at 23.3°C. One 0.045 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Extracellular matrix binding protein FG-repeats (rEmbp6599)
Mol. type   Protein
Organism   Staphylococcus epidermidis
Olig. state   Other
Mon. MW   85.5 kDa
 
UniProt   Q5HPA2
Sequence   FASTA
 
PDB ID   6GV5