SAXS Measurements of the SAM2 Riboswitch

Alex Plumridge.

SASDJA7 – SAM2 Riboswitch 0.5 mM MgCl2

S-adenosylmethionine riboswitch II
MWexperimental 20 kDa
MWexpected 17 kDa
log I(s) 6.50×10-3 6.50×10-4 6.50×10-5 6.50×10-6
S-adenosylmethionine riboswitch II small angle scattering data  s, nm-1
ln I(s)
S-adenosylmethionine riboswitch II Guinier plot ln 6.50×10-3 Rg: 3.2 nm 0 (3.2 nm)-2 s2
(sRg)2I(s)/I(0)
S-adenosylmethionine riboswitch II Kratky plot 1.104 0 √3 sRg

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of SAM2 Riboswitch 0.5 mM MgCl2 in 20 mM NaMOPS, 20 mM NaCl, 20 μM EDTA, 0.5 mM MgCl2, pH 7 were collected on the G1 beam line at the Cornell High Energy Synchrotron Source (CHESS) storage ring (Ithaca, NY, USA) using a Pilatus 100K detector at a sample-detector distance of 1.7 m and at a wavelength of λ = 0.11 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.5 and 1.8 mg/ml were measured . 20 successive 5 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.

Cell temperature = UNKNOWN. Storage temperature = UNKNOWN

S-adenosylmethionine riboswitch II (SAM-II)
Mol. type   RNA
Olig. state   Monomer
Mon. MW   16.9 kDa
Sequence   FASTA