Lactococcus Phage ASCC Phi28 gp11

Mark White.

SASDJB6 – Lactococcus phage Phi28 apo-gp11 encapsidation protein

gp11 encapsidation protein
MWI(0) 368 kDa
MWexpected 446 kDa
log I(s) 3.66×105 3.66×104 3.66×103 3.66×102
gp11 encapsidation protein small angle scattering data  s, nm-1
ln I(s)
gp11 encapsidation protein Guinier plot ln 3.67×105 Rg: 5.3 nm 0 (5.3 nm)-2 s2
(sRg)2I(s)/I(0)
gp11 encapsidation protein Kratky plot 1.104 0 √3 sRg
p(r)
gp11 encapsidation protein pair distance distribution function Rg: 5.3 nm 0 Dmax: 16.3 nm

Data validation

Fits and models

log I(s)
 s, nm-1
gp11 encapsidation protein OTHER model
log I(s)
 s, nm-1
gp11 encapsidation protein DAMMIF model
gp11 encapsidation protein DAMMIF model
SAXS data from solutions of Lactococcus phage Phi28 apo-gp11 encapsidation protein in 100 mM NaCl, 25 mM HEPES, pH 7.4 were collected using a Pilatus 100K detector at a wavelength of λ = 1.5418 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 10°C. 12 successive 3600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Sample detector distance = UNKNOWN

gp11 encapsidation protein (gp11)
Mol. type   Protein
Organism   Lactococcus phage Phi28
Olig. state   Decamer
Mon. MW   44.6 kDa
 
UniProt   B1ABI1
Sequence   FASTA
 
PDB ID   7JQP