| MWI(0) | 30 | kDa |
| MWexpected | 33 | kDa |
| VPorod | 47 | nm3 |
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log I(s)
3.53×102
3.53×101
3.53×100
3.53×10-1
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s, nm-1
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Unique Structural Features of Mammalian NEIL2 DNA Glycosylase Prime Its Activity for Diverse DNA Substrates and Environments.
Eckenroth BE, Cao VB, Averill AM, Dragon JA, Doublié S, Structure (2020) Europe PMCSASDJC4 – Endonuclease VIII - Like 2 from Monodelphis domestica (size exclusion chromatography SAXS) MdoNEIL2 cut (Δ67-133)
Nei like DNA glycosylase 2 (Δ67-133)|
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Fits and models
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Synchrotron SAXS
data from solutions of
Endonuclease VIII - Like 2 from Monodelphis domestica (size exclusion chromatography SAXS) MdoNEIL2 cut (Δ67-133)
in
25 mM Bis-Tris, 150 mM NaCl, 2% glycerol, 1 mM TCEP, pH 8
were collected
on the
12.3.1 (SIBYLS) beam line
at the Advanced Light Source (ALS) storage ring
(Berkeley, CA, USA)
using a Pilatus3 X 2M detector
at a wavelength of λ = 0.103 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 70.00 μl sample
at 10 mg/ml was injected at a 0.50 ml/min flow rate
onto a Shodex KW-800 series column
at 25°C.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Data collected with in-line SEC |
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