| MWexperimental | 139 | kDa |
| MWexpected | 155 | kDa |
| VPorod | 240 | nm3 |
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log I(s)
4.50×10-2
4.50×10-3
4.50×10-4
4.50×10-5
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s, nm-1
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A molecular mechanism for LINC complex branching by structurally diverse SUN-KASH 6:6 assemblies.
Gurusaran M, Davies OR, Elife 10 (2021) Europe PMCSASDJC5 – LINC complex between the SUN domain of SUN1 and KASH domain of Nesprin-4 - SUN1-KASH4 6:6 complex
SUN domain-containing protein 1Nesprin-4
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Fits and models
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Synchrotron SAXS data from solutions of the Nesprin-4-KASH/SUN1 6:6 complex in 20 mM Tris pH 8.0, 150 mM KCl, were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Pilatus 2M detector at a wavelength of λ = 0.095 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 17 mg/ml was injected at a 0.50 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 31 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN |
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