| MWexperimental | 3 | kDa |
| MWexpected | 4 | kDa |
| VPorod | 3 | nm3 |
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log I(s)
1.10×10-2
1.10×10-3
1.10×10-4
1.10×10-5
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s, nm-1
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Towards improved understanding of intersubunit interactions in modular polyketide biosynthesis: docking in the enacyloxin IIa polyketide synthase.
Risser F, Collin S, Dos Santos-Morais R, Gruez A, Chagot B, Weissman KJ, J Struct Biol :107581 (2020) Europe PMCSASDJE2 – Beta-ketoacyl synthase (Bamb_5925) C-terminal docking domain
Beta-ketoacyl synthase Bamb_5925|
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Fits and models
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Synchrotron SAXS data from solutions of Bamb_5925 CDD in 20 mM Tris-HCl, 200 mM NaCl, 5% glycerol, pH 7.5 were collected on the SWING beam line at SOLEIL (Saint-Aubin, France) using a AVIEX PCCD170170 detector at a sample-detector distance of 1.8 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 30 mg/ml was injected at a 0.10 ml/min flow rate onto a Agilent Bio SEC-3, 100 Å column at 15°C. 100 successive 0.750 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The protein samples were injected using the online automatic sample changer into a pre-equilibrated HPLC-coupled size-exclusion chromatography column. |
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