| MWexperimental | 73 | kDa |
| MWexpected | 74 | kDa |
| VPorod | 116 | nm3 |
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log I(s)
2.29×104
2.29×103
2.29×102
2.29×101
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s, nm-1
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Mechanism of activation and regulation of deubiquitinase activity in MINDY1 and MINDY2.
Abdul Rehman SA, Armstrong LA, Lange SM, Kristariyanto YA, Gräwert TW, Knebel A, Svergun DI, Kulathu Y, Mol Cell (2021) Europe PMCSASDJE3 – Ubiquitin carboxyl-terminal hydrolase, MINDY2 (C266A mutant), bound to penta-ubiquitin
Ubiquitin carboxyl-terminal hydrolase C266A mutantPolyubiquitin-C
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Fits and models
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Synchrotron SAXS data from solutions of MINDY2 (C266A mutant) bound to penta-ubiquitin in 20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.12 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 75.00 μl sample at 17.1 mg/ml was injected at a 0.50 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 3000 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The experimental MW was determined from Porod volume. |
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