Improving data quality and expanding BioSAXS experiments to low-molecular-weight and low-concentration protein samples.

Castellví A, Pascual-Izarra C, Crosas E, Malfois M, Juanhuix J, Acta Crystallogr D Struct Biol 76(Pt 10):971-981 (2020) Europe PMC

SASDJF3 – Aldo-keto reductase family 1 member B1 (AKR1B1) in the presence of 100 mM 5-methyl uridine

Aldo-keto reductase family 1 member B1
MWexperimental 36 kDa
MWexpected 36 kDa
VPorod 52 nm3
log I(s) 4.44×10-3 4.44×10-4 4.44×10-5 4.44×10-6
Aldo-keto reductase family 1 member B1 small angle scattering data  s, nm-1
ln I(s)
Aldo-keto reductase family 1 member B1 Guinier plot ln 4.44×10-3 Rg: 2.0 nm 0 (2.0 nm)-2 s2
(sRg)2I(s)/I(0)
Aldo-keto reductase family 1 member B1 Kratky plot 1.104 0 3 sRg
Dmax: 6.5 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from a solution of 7.00 mg/ml human Aldose Reductase (AKR1B1) in 20 mM Tris, 250 mM NaCl, 100 mM 5-methyl uridine was collected on the BL11-NCD-SWEET beam line at ALBA Synchrotron using a Pilatus 1M photon-counting detector. The beam at sample position was 0.566 mm (horizontal) × 0.420 mm (vertical), the incident photon flux was 1.08×10e12 photons/s at 12.4 keV (λ =0.0999 nm) and the sample to detector distance was 2.460 m. 2000 successive 0.1 second frames were collected and data were normalized to the intensity of the transmitted beam and radially averaged. The current buffer-subtracted scattering profile corresponds to the average of 370 collected frames. The absorbed dose by the sample was 32.19 kGy.

Sample temperature: 25°C. CAUTION! The scattering intensities do not have associated errors.

Aldo-keto reductase family 1 member B1 (AKR1B1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   35.9 kDa
 
UniProt   P15121 (1-316)
Sequence   FASTA