Structural Characterization of Plant Metallothionein

Dima Molodenskiy.

SASDJF6 – Apo-full-length protein titrated with Cd(II) ions

Metallothionein-like protein 2
MWexperimental 10 kDa
MWexpected 8 kDa
VPorod 13 nm3
log I(s) 8.50×10-3 8.50×10-4 8.50×10-5 8.50×10-6
Metallothionein-like protein 2 small angle scattering data  s, nm-1
ln I(s)
Metallothionein-like protein 2 Guinier plot ln 8.50×10-3 Rg: 2.6 nm 0 (2.6 nm)-2 s2
(sRg)2I(s)/I(0)
Metallothionein-like protein 2 Kratky plot 1.104 0 √3 sRg
p(r)
Metallothionein-like protein 2 pair distance distribution function Rg: 2.6 nm 0 Dmax: 10 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Apo-full-length protein titrated with Cd(II) ions Rg histogram Rg, nm
Metallothionein-like protein 2 EOM/RANCH model
Metallothionein-like protein 2 EOM/RANCH model
Metallothionein-like protein 2 EOM/RANCH model
Metallothionein-like protein 2 EOM/RANCH model
log I(s)
 s, nm-1
Metallothionein-like protein 2 DAMMIF model
Synchrotron SAXS data from solutions of Apo-full-length protein titrated with Cd(II) ions in 10 mM Tris, 50 mM NaCl + 1mM TCEP (apoMT), pH 7.4 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 20°C. 80 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Metallothionein-like protein 2 (holoMT2)
Mol. type   Protein
Organism   Cicer arietinum
Olig. state   Monomer
Mon. MW   7.8 kDa
 
UniProt   Q39459 (2-79)
Sequence   FASTA