Structural Characterization of Plant Metallothionein

Dima Molodenskiy.

SASDJG6 – The separated apo-linker region peptide

The separated apo-linker region peptide from the plant Cicer arietinum
MWexperimental 1 kDa
MWexpected 4 kDa
VPorod 1 nm3
log I(s) 1.36×10-3 1.36×10-4 1.36×10-5 1.36×10-6
The separated apo-linker region peptide from the plant Cicer arietinum small angle scattering data  s, nm-1
ln I(s)
The separated apo-linker region peptide from the plant Cicer arietinum Guinier plot ln 1.36×10-3 Rg: 1.4 nm 0 (1.4 nm)-2 s2
(sRg)2I(s)/I(0)
The separated apo-linker region peptide from the plant Cicer arietinum Kratky plot 1.104 0 √3 sRg
p(r)
The separated apo-linker region peptide from the plant Cicer arietinum pair distance distribution function Rg: 1.4 nm 0 Dmax: 6.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
The separated apo-linker region peptide Rg histogram Rg, nm
The separated apo-linker region peptide from the plant Cicer arietinum EOM/RANCH model
The separated apo-linker region peptide from the plant Cicer arietinum EOM/RANCH model
The separated apo-linker region peptide from the plant Cicer arietinum EOM/RANCH model
log I(s)
 s, nm-1
The separated apo-linker region peptide from the plant Cicer arietinum DAMMIF model
Synchrotron SAXS data from solutions of The separated apo-linker region peptide in 10 mM Tris, 50 mM NaCl, pH 7.4 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.55 mg/ml was measured at 20°C. 40 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The separated apo-linker region peptide from the plant Cicer arietinum (Apo-linker)
Mol. type   Protein
Organism   Cicer arietinum
Olig. state   Monomer
Mon. MW   4.3 kDa
Sequence   FASTA