Zeronian MR,
Klykov O,
Portell I de Montserrat J,
Konijnenberg MJ,
Gaur A,
Scheltema RA,
Janssen BJC,
Proc Natl Acad Sci U S A
118(30)
(2021)
Europe PMC
SASDJG8 – Monomeric neurogenic locus notch homolog protein 1 (Notch1) full ectodomain
Synchrotron SAXS
data from solutions of
Monomeric neurogenic locus notch homolog protein 1 (Notch1) full ectodomain
in
20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, pH 7.4
were collected
on the
BM29 beam line
at the ESRF storage ring
(Grenoble, France)
using a Pilatus 1M detector
at a sample-detector distance of 2.9 m and
at a wavelength of λ = 0.09919 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 500.00 μl sample
at 1.7 mg/ml was injected
onto a GE Superose 6 Increase 10/300 column
at 19°C.
2000 successive
2 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The expected molecular weight of the protein quoted above excludes the contribution of N- and O-linked glycans. The total expected molecular weight of the protein in a monomeric state is 210 kDa (protein, 183 kDa plus glycans, 27 kDa).
s, nm-1
